Tricks to eliminate non-specific binding of proteins to amylose r esin, any ?

Serial # 19781010 tvink at xs4all.nl
Sat Jan 19 06:26:22 EST 2002


Elute your complex with maltose ?
Preblock your column ?

grtz Tom

On 17 Jan 2002 16:32:55 -0000, khadkad at mail.nih.gov ("Khadka, Deepak
(NICHD)") wrote:

>
>I am performing binding assay of some test proteins with MBP-fusion protein.
>When I try to purify the mixture by using amylose resin, most of the test
>proteins gets stuck to the resin. My buffer (both for binding and washing)
>is 50 mM PIPES (pH 7.0), 100 mM NaCl and 1 mM EDTA. Playing with the salt
>concentration and adding BSA did not help. Any suggestions ?
>
>Deepak
>NICHD/NIH
>
>---




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