Tricks to eliminate non-specific binding of proteins to amylose resin, any ?
bier13 at mail.com
Sat Jan 19 13:25:58 EST 2002
I purified like five different mpb fusie proteins with amylose resin
and it worked well.
Did you try glucose into your bacterie grow medium??
To wash i use this buffer (for 100ml)
25 ml 0,2 M KHPO4 pH 7,4
4 ml 5 M Nacl
200 ul 0,5 EDTA pH 8,0
70 ml MQ
And for the elution a use the excact same but than with 10 mM Maltose.
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