a question on how to improve solubility of my protein

Guoping Su no at no.edu
Mon Jan 21 21:22:58 EST 2002


Hi, all,

I am looking for methods to improve solubility of a protein. I have the gene
built in a vector with his-tag on both terminals. but whenever I induce the
expression (in E.coli), the protein goes into inclusion body. Do you have
better ideas to possibly get soluble protein?
Thanks very much for your time.








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