a question on how to improve solubility of my protein
legatek at mcmail.cis.mcmaster.ca
Tue Jan 22 12:09:36 EST 2002
On Mon, 21 Jan 2002, Guoping Su wrote:
> I am looking for methods to improve solubility of a protein. I have the gene
> built in a vector with his-tag on both terminals. but whenever I induce the
> expression (in E.coli), the protein goes into inclusion body. Do you have
> better ideas to possibly get soluble protein?
> Thanks very much for your time.
What I find helps is to grow the bugs at a lower temperature (someone
around here goes as low as 16C). The idea is to slow translation so that
your protein has more time to fold in a soluble manner such that it
doesn't get sequestered in inclusion bodies. Good luck.
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legatek at mcmaster.ca Kyle Legate legatek at hotmail.com
Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
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