imidazole vs. histidine

Nikolai Scherbak nikolai.scherbak at nat.oru.se
Wed Jan 23 07:31:28 EST 2002


Dear collegues!
I am working with purification of a recombinant His-tagged protein 
using Ni-NTA column. I am wondering if there is any difference in 
using imidazole vs. L-histidine for elution? Imidazole is cheaper 
but, as I heard, it can influence activity of my protein, so 
L-histidine is more recommended. Can it be so?
Thanx in advance

Nikolai
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Nikolai Scherbak
Orebro University
Fakultetsgatan 1
S-70182, Orebro, SWEDEN
tel.+46-19-301034
fax +46-19-303566

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