gel filtration vs dialysis

Yong Jiang yjiang at ucsd.edu
Thu Jan 24 20:22:39 EST 2002


I wouldn't do that, because I don't trust the mw cut of dialysis tubing, you
can not get rid of the bacterial homolog completely.
yong
"Dominic-Luc Webb molmed" <domweb at mbox.ki.se> wrote in message
news:Pine.GSO.4.43.0201081622320.22958-100000 at mbox.ki.se...
>
> I want to get rid of proteases, mainly from an E coli
> expression system in which I am expressing an 86 KD
> protein (mammalian). The bacterial homolog is 32 KD
> and I also want this removed. This seems like classical
> job for gel filtration, but I am thinking that maybe
> the Spectra/por 6 or 7 RC dialysis tubing with 50 KD MWCO
> might work better (like preserving the volume) or
> maybe it would give better/faster separation. My
> understanding from reading is that bacterial proteases
> are typically very small, and well below this 50 KD
> MWCO.
>
> Could someone please tell me if this is not such a
> clever idea and why or why not?
>
>
> Cheers,
>
>
> Dominic
>
> North 59 37' 30"
> East  17 48' 10"
>





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