stabilizing enzyme during storage

Peter Cherepanov peter.cherepanov at uz.kuleuven.ac.be
Mon Jul 1 05:31:36 EST 2002


It is really not good to keep enzymes frozen at -20C.
I red somewhere it has something to do with properities of salt solutions at
these temperatures.

Either add 50% glycerol and -20C (does not freeze).
or add 10% glycerol, snap freeze and keep at -70C.

phosphate buffer is not good for freezing anyway.
Especially slow freezing - it will give unstable (too acid?) pH as one of
the salts can precipitate.

Peter

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