PROBLEM WITH RP-HPLC PURIFICATION OF PEPTIDE

Roger Murphy roger.murphy at ludwig.edu.au
Tue Jul 2 20:31:32 EST 2002


This peptide is a perfect candidate for ion exchange purification, something a 
lot of peptide chemists seem to forget about nowadays.

In article <20020702215153.21164.qmail at ww02.hostica.com>, shibbu_r at yahoo.com 
wrote:
>
>I HAVE A PROBLEM WITH THE PURIFICATION OF 26 AMINOACID PEPTIDE PREPARED BY
> SOLID PHASE PEPTIDE SYNTHESIS. THE SEQUENCE IS
>
>    NH2-CGGGEGGGEGGGEGGGEGGGEGGGEG-CONH2
>
>THEY ARE PREPARED BY FRAGMENT CONDENSATION OF TETRA PEPTIDES.  
>
>
>PEPTIDE IS NOT VISIBLE AS A PEAK IN TFA(PH2) AND AMMONIUM ACETATE (PH 5.5)
> BUFFERS. IN AMMOUNIUM BICARBONATE BUFFER (PH 7) IN C18 9.4*250mm  COLUMN IT
> HAS RETENTION TIME OF ABOUT 3.5MINUTES AND IT COELUTES WITH OTHER DELETION
> PEPTIDES OF MY SOLID PHASE SYNTHESIS.
>
>
>ANY SUGGESTIONS ON DIFFERENT BUFFER OR COLUMN CONDITIONS???
>
>
>THANKS,
>
>SIVAKUMAR 
>http://www.biowww.net/index.php/forum/forumlist/1/



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