Help: Protein Determination w/out spectrometer possible?
Frank O. Fackelmayer
Frank at Fackelmayer.de
Wed Jul 10 11:29:24 EST 2002
well, it depends on how accurate the determination must be. Given the
inherent inaccuracy of all methods due to protein-to-protein variations,
I suggest you use a colorimetric test (such as Bradford's) and good
standard samples determined in parallel.
The test works well between 1 and 12ug protein (e.g. dissolved in 1ml of
water or buffer). I do standards of 0, 2, 4, 6, 8, 10, and 12ug of BSA,
which give a nice range from brown to blue. Given your sample is in the
same range of concentration, a visual inspection of the assay is
sufficient to say what the approximate concentration is.
> Hi all.
> I would like to search for a method to determine protein (crude or
> otherwise) in soybean meal, and samples similar to soybean meal.
> I work in a tiny tiny lab, mainly doing oil and fat analyses. This
> would be a new test for us. We have no spectrometer. The more
> sophisticated equipments that we have is a Lovibond tintometer (RYB
> visual colormeter), and a muffle furnace.
> Does anybody have an idea if this is even possible?
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