help

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Tue Jul 23 20:58:01 EST 2002


There's a number of methods that can be used for protein extraction out of
electrophoresis gel matrices. They range from crushing the gel & soaking, to
electroelution, to digestion of the matrix with chemicals (e.g. potassium
iodide-agarose) or enzymes. It all depends on what do you need the protein
FOR. If it's mass-spec then some techniques are better than others (for
example, MS really does not like polyacrylamide fragments which are common
contaminants in many elution techniques).

A.G.E.

"Yuyuan Guo" <yuyuan at students.wisc.edu> wrote in message
news:ahiic3$pdi$1 at news.doit.wisc.edu...
> Does somebody know how to isolate proteins from PAGE Gel after
> electrophoresis? Any information is welcome.
>
> I hope to get a protocol.
>
> Thanks.
>
> Sincerely
>
> Guo
>
>





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