How to generate truncated proteins?

benjaminely at benjaminely at
Fri Jul 26 05:55:39 EST 2002

Dear All,
I am trying to determine the protein solubility of truncated proteins.  Thus, am wondering for different methods used for invitro generation of truncated proteins.  Could someone please comment on some methods?  

Does someone has any idea of generating nonsense mutations using PCR so that truncated protein could be generated by translation of the non sense mRNA thus generated ? 

Any comment will be greatly appreciated.
Thank you,

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