How to generate truncated proteins?

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Fri Jul 26 18:32:29 EST 2002


On 26 Jul 2002 benjaminely at hotmail.com wrote:

> Dear All,
> I am trying to determine the protein solubility of truncated proteins.  Thus, am wondering for different methods used for invitro generation of truncated proteins.  Could someone please comment on some methods?
>
If there are restriction sites within the coding sequence you can cut the
plasmid with restriction enzymes and translate the truncated DNA. At the
end of the translation you can release the truncated product from the
ribosome with 1mM puromycin and some EDTA.

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legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

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