STREP-Tag purification

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Fri Jun 14 22:05:03 EST 2002


Depends what you use the protein for :) Just as any tag, there are problems.
For example we could not crystallize one of our proteins with a C-terminal
STREP tag, even though it'd crystallize just fine with Argx5, Hisx6 and (!)
the biotin-acceptor peptide.

STREP tag is definitely NOT my first choice, in general.
A.G.E.
"Philipp Wechner" <philipp.wechner at uibk.ac.at> wrote in message
news:3D09C559.EEA9E8CD at uibk.ac.at...
> Hi dear newsgroup....
>
> Does anybody have experience with the STREP tag? Is it efficient to
> purify taged proteins? any problems with urea?
>
>





More information about the Proteins mailing list