purification of FC-fusion protein

beatrice mautino at dba.unito.it
Thu Mar 7 06:05:17 EST 2002

Hi all,
I'm trying to purify a Fc-fusion protein using protein-G beads (from
Santa Cruz) without good results.
I directly add the beads to the conditioned media (diluted 1:4 in
water), incubate for an hour at 4°C rotating, wash twice with PBS and
then elute with 0,1M Na-citrate pH 3.5.
Well, the beads are unable to bind the fusion prot, Why?
Have you got some suggestions?
thanks in advance,

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