how to make a protein soluble

Artem Evdokimov AEVDOKIMOZ at
Thu Mar 14 20:28:40 EST 2002

Fusion to other proteins, most notably MBP. Expression at lower
temperatures. Expression in altered strains of e. coli, or expression into
the periplasm. Expression in other organisms. Structure-based engineered
mutations. Goat sacrifice.

Hope this helps.

"Su" <roxx0005 at> wrote in message
news:20020314180306.9943.qmail at
> I'm currently working with the pex7 gene in pet 28a vector.
> i ran an sds gel and found that the protein of interest is located in the
> insoluble portion of the cell.. my question is what are some of the ways I
> can make this protein soluble?  Thank you.
> <>

More information about the Proteins mailing list