Measuring peptide concentration

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Sun Mar 17 18:09:39 EST 2002


The Thr, obviously, should read Tyr.

A.

"Artem Evdokimov" <AEVDOKIMOZ at cinci.rr.com> wrote in message
news:pm9l8.163960$Hu6.37747719 at typhoon.neo.rr.com...
> Well.
>
> It depends if you have Phe, Thr, Trp. If you do then calculate the
> theoretical A280 ratio and measure unfolded in 6M guanidine. This is
> reasonably close to reality. If you wish to get more accurate then you can
> use any of the colorimetric assays. If you wish to get even more accurate
> then you should use quantitative N-terminal sequencing or total nitrogen
> analysis.
>
> Cheers,
>
> A.G.E.
> "JB" <j.bukczynskiDELETE_ME at utoronto.ca> wrote in message
> news:b87a9ugslasbigp8lm4dhkqc4p0soe0se7 at 4ax.com...
> > Hi,
> >
> > Does anyone know how to measure peptide concentration using a
> > spectrophotometer? I have it dissolved in 10% DMSO in PBS. Any help
> > would be appreciated. Thanks,
> >
> > Jacob
>
>





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