Aminopeptidase???

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Thu Mar 28 18:19:34 EST 2002


If you only wanted to remove one, then Met-Pro is very efficiently cleaved
by methionine aminopeptidase. Met-met-pro isn't so hot though. Would it be
easier to re-clone the thing ?

A.G.E.
""Lipari, Francesco"" <Francesco.Lipari at DSM.COM> wrote in message
news:896508DC6B0ED511A90400508B6838EE48DFDB at camnt11ntms002.mnt11.dsm-group.c
om...
> Hi,
> I am trying to remove two amino acids from the N-terminus of my favorite
> protein using aminopeptidases. The sequence of the protein is XaaXaaPro...
>
> Apparently, it will be difficult to perform this because commercial
> aminopeptidases do not cleave at the Xaa-Pro bond.
>
> I am looking for a solution to this very interesting problem.
>
> Thank you,
>
> Francesco Lipari, Ph.D.
> Senior Scientist Analytical Development
> DSM Biologics
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