streptavidin purification

michael durney nitrogen_15 at yahoo.co.uk
Mon May 6 06:26:30 EST 2002


hello all

can anyone give me some general advice on purifying
biotin tagged proteins with streptavidin?

ie: do the conditions have to be harsh to elute the
protein? what types of buffers will work?

a literature reference would be appreciated.

thanks in advance!

mike

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