streptavidin purification

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Tue May 7 19:29:36 EST 2002


Pubmed with targeted keywords should be good. A bunch of companies have
published protocols...

That kind of stuff is also in most books on protein production :)

Good luck. I am sure people here would be happy to help you if you run into
specific problems, but this kind of research is basically your homework :)

A.G.E.
"michael durney" <nitrogen_15 at yahoo.co.uk> wrote in message
news:20020506112624.31639.qmail at web11204.mail.yahoo.com...
> hello all
>
> can anyone give me some general advice on purifying
> biotin tagged proteins with streptavidin?
>
> ie: do the conditions have to be harsh to elute the
> protein? what types of buffers will work?
>
> a literature reference would be appreciated.
>
> thanks in advance!
>
> mike
>
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