aggregation in dialysis

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Wed May 8 19:00:47 EST 2002


What's the salt concentration ? Did you try detergents yet ?

A.G.E.
"Mariella" <parisi at fis.unipr.it> wrote in message
news:fad5fa87.0205072344.5b20a4eb at posting.google.com...
> I used continous flow dialysis against buffer (TRIS or Phosphate
> buffer PH 7) for changing buffer. The time isn't important.
>
> "Artem Evdokimov" <AEVDOKIMOZ at cinci.rr.com> wrote in message
news:<9g_B8.58555$Ez5.14761633 at typhoon.neo.rr.com>...
> > Detergents ? Continuous flow dialysis ? Dialysis via ultracentrifugation
?
> >
> > it really depends on the details - how long do you dialyze, against what
> > (and from what) etc.
> >
> > A.G.E.
> > "Mariella" <parisi at fis.unipr.it> wrote in message
> > news:fad5fa87.0205070655.3e5eafde at posting.google.com...
> > > Hi everybody!
> > > I've a problem. The protein I study gets aggregated when I put it in
> > > dialysis. I've tried many things: to change the buffer, collodion
> > > bags,
> > >  to add EDTA or Glycerol or Salt. But nothing.
> > > By X-ray it seems that nothing binds it, and so I've rejected the idea
> > > that
> > > the protein loses a ligand in dialysis.
> > > The protein is the porcine Odorant Binding Protein (a Lipocalin): its
> > > analogous one ( that one extract from bovine) doesn't have this
> > > behaviour.
> > > What can I do?
> > >
> > > Thanks
> > >
> > > Mariella Parisi





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