tlyms at sol.dk (Thomas) schrieb:
> Help needed
>> I want to replace beta-mercaptoethanol with cysteine as antioxidant in
> my buffers.
Do you have a specific reason for that?
> Have you got any experience?
No, I just used beta-mercaptoethanol, DTE (DiThioErythritol) and DTT
(DiThioThreitol), and I know people use GSSH.
> Any thing to be take into consideration?
- redox potential
- pKa which has a great effect on the kinetics
> But I don't really see running SETI at Home as practical as Folding at Home. What,
> exactly, would be the benefit of finding intelligent aliens on the other side
> of the galaxy?
Maybe they're broadcasting the principles of protein folding... [from bionet.*]