Replacing beta-mercaptoethanol with cysteine

vsghole at chem.unipune.ernet.in vsghole at chem.unipune.ernet.in
Sat May 18 04:07:50 EST 2002


Dear Thomas
	Pl.let us know about the purpose for which you want to use
Cysteine? But Cysteine provides excellent and reversible reducing
conditions while working with oxidoreductases.
	thanks
	V.S.Ghole
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On 9 May 2002, Thomas wrote:

> Help needed
> 
> I want to replace beta-mercaptoethanol with cysteine as antioxidant in
> my buffers. Have you got any experience? Any thing to be take into
> consideration? What concentrations of cysteine do one need to replace
> 1 mM and 10 mM beta-mercaptoethanol?
> 
> Cheers
> 
> Thomas =:-)
> 

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Dr.V.S.Ghole, M.Sc.,Ph.D.
Reader in Bichemistry,
Division of Biochemistry,
Department of Chemistry,
And
Head Of the Department of Environmental Sciences,
University of Pune, Pune-411007, India.
Tel.#   91-(020)-569 6061 (Ext.1231 Chemitry)
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