Replacing beta-mercaptoethanol with cysteine

Miriam Hirshberg mh270 at bioc.cam.ac.uk
Sat May 18 04:39:47 EST 2002


Dear V.S.Ghole,

 both DTT and bME go off with time, and if you use them in
crystallization trails for example, this process might be faster than the
actual nucleation and crystal growth. Would you recommend Cys instead??

Thanks, Miri

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   Miri Hirshberg               e-mail: m.hirshberg at mole.bio.cam.ac.uk
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On 18 May 2002 vsghole at chem.unipune.ernet.in wrote:

> Dear Thomas
> 	Pl.let us know about the purpose for which you want to use
> Cysteine? But Cysteine provides excellent and reversible reducing
> conditions while working with oxidoreductases.
> 	thanks
> 	V.S.Ghole
> ===========================================
>
> On 9 May 2002, Thomas wrote:
>
> > Help needed
> >
> > I want to replace beta-mercaptoethanol with cysteine as antioxidant in
> > my buffers. Have you got any experience? Any thing to be take into
> > consideration? What concentrations of cysteine do one need to replace
> > 1 mM and 10 mM beta-mercaptoethanol?
> >
> > Cheers
> >
> > Thomas =:-)
> >
>
> --
> *******************************************************************************
> Dr.V.S.Ghole, M.Sc.,Ph.D.
> Reader in Bichemistry,
> Division of Biochemistry,
> Department of Chemistry,
> And
> Head Of the Department of Environmental Sciences,
> University of Pune, Pune-411007, India.
> Tel.#   91-(020)-569 6061 (Ext.1231 Chemitry)
> FAX #   91-(020)-569 1728 (Chemistry)
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> FAX #   91-(020)-569 0087 or 569 3899 ( please write Attn.: Dr.V.S.Ghole)
> *******************************************************************************
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> ---
>
>

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