Efficiency of electroelution?

dustjohn at hotmail.com dustjohn at hotmail.com
Sat Nov 2 16:30:59 EST 2002


Just wondering if electroeluting proteins (i.e. cutting out protein band from native PAGE and passing current through the gel slice) is a decent way of isolating pure protein?  Does this method sacrifice enzymatic activity for purity or can the enzymatic activity be saved?  (I hear one must avoid production of free radicals within the buffer?)
Thanks,
Dustin


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