Gel sticks to membrane

obrarzona at obrarzona at
Thu Nov 7 15:15:58 EST 2002

Does anyone know how to avoid the gel sticking to the membrane while blotting?  I'm using a 4-15% continous gradient ready gel.  About 1/3 (top part) of the gel sticks.  Also, if it happens again, what's the best way to get it off?  It's very sticky.  I tried rinsing it with wash buffer, but it's still there.  Thanks!

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