IgM immunoprecipitation

get real allen at NOSPAM.icm.umontreal.ca
Tue Nov 12 23:38:10 EST 2002


On Tue, 12 Nov 2002 1:28:03 -0500, D.K. wrote
(in message <aqq71o$l8k$1 at news.doit.wisc.edu>):

>  Jos Broers <Jos.Broers at molcelb.unimaas.nl> wrote:
>> Dear All,
>> Is it possible to perform an immunoprecipitation with protein-A 
>> sepaharose beads and a mouse IgM antibody?
>> I doubt whether I get any binding.
> 
> You are right, you won't have binding.
> 
> Check Pierce catalog - if i remember correctly (not sure)
> there is something (lectin?) that binds IgM specifically.
> 
> DK

Try Protein G agarose or protein A/G agarose




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