histidine tagging

James Bassuk bassuk at u.washington.edu
Sun Nov 17 17:58:29 EST 2002


On 29 Oct 2002, Nora Plesofsky wrote:

> I wish to tag proteins that I have engineered to be expressed in my
> organism. It seems to me that hexahistidine tagging would serve two
> purposes, i.e. allowing visualization of the protein by immunoblotting
> and also affinity isolation of the protein with attendant proteins. I
> am asking if others have had experience with antibody reactivity to the
> histidine tag and with background reactivity. Is there another tag that
> has served this purpose possibly better?
>
> Thanks for your help.
>
> Nora Plesofsky
> nora at biosci.cbs.umn.edu
>
> ---
>

The C-terminal anti-His tag IgG from Invitrogen is the best on the market.
It gives no background on westerns and works great on immunohistochemistry
stained sections.

Jim




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