protein precipitation

janosch groening janosch.groening at ioez.tu-freiberg.de
Fri Nov 22 01:56:02 EST 2002


Hi Sigrid,
if you just want to concentrate the protein in the solution you could also try it by using a SpeedVac. This works without precipitation, though.
janosch

s.hannier at abdn.ac.uk schrieb:

> I would like to concentrate a sample of proteins with less than 1 ug  (aout 900ng) in 1ml. What is the best precipitation method to use for a maximum recovery? I have heard about deoxycholate/TCA but it seems that recovery is 50% , which is not very good. Any advice?
>
> Many thanks,
>
> Sigrid
>
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
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