Protein purification after in vitro transcription/translation using Roche RTS100

aennsche at yahoo.com aennsche at yahoo.com
Tue Nov 26 09:19:39 EST 2002


Hello!
did anyone use the protein in vitro transcription / translation system RTS100 by Roche. i would 
like to express my kinase (which is not possible in bacteria, yeast and plant cells) in order to do 
kinase assays and pulldowns. i was wondering if the yield is high enough to be able to purify the 
desired protein and then use it.

http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0



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