basic proteins--help

Baichen Zhang Zhang at mpimp-golm.mpg.de
Fri Oct 4 10:43:52 EST 2002


Could anyone suggest some points on estimation of isoelectric point of basic proteins by IEF? I find the IEF strips available mostly have a given PI range of below 10.

Thanks in advance,

Baichen

-----Original Message-----
From: mamasuda at gan2.res.ncc.go.jp [mailto:mamasuda at gan2.res.ncc.go.jp]
Sent: Freitag, 4. Oktober 2002 09:14
To: proteins at hgmp.mrc.ac.uk
Subject: Fc-fusion protein



Does anybody know how to make a protein fused to the Fc portion of human IgG ? I know that vectors containing GFP, V5, His, or flag-tag are commercially available. But, I have not seen any commercially available vector containing Fc portion of human IgG. Do I have to obtain cDNA coding IgG Fc first?

http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
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