Zhang at mpimp-golm.mpg.de
Fri Oct 4 10:43:52 EST 2002
Could anyone suggest some points on estimation of isoelectric point of basic proteins by IEF? I find the IEF strips available mostly have a given PI range of below 10.
Thanks in advance,
From: mamasuda at gan2.res.ncc.go.jp [mailto:mamasuda at gan2.res.ncc.go.jp]
Sent: Freitag, 4. Oktober 2002 09:14
To: proteins at hgmp.mrc.ac.uk
Subject: Fc-fusion protein
Does anybody know how to make a protein fused to the Fc portion of human IgG ? I know that vectors containing GFP, V5, His, or flag-tag are commercially available. But, I have not seen any commercially available vector containing Fc portion of human IgG. Do I have to obtain cDNA coding IgG Fc first?
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