buffer and metals

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Wed Oct 9 19:41:10 EST 2002


On Wed, 09 Oct 2002 16:04:34 +0200, Philipp Wechner
<philipp.wechner at uibk.ac.at> wrote:

>As far as I know Tris does not interact with metal ions. I don't know it for
>Hepes.

IIRC, low metal binding was one of the features that Good looked for
in designing  zitterionic buffers such as HEPES.

>Phosphate-buffer should also be ok

You sure? Phosphate can bind to Ca and Mg pretty strongly.

Nick 

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu



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