silver stain problem

beismann at beismann at
Thu Oct 10 09:24:32 EST 2002


I have done silver stains of SDS gels many times before but last time something strange occured: I got an inverse stain with dark background and light protein bands (altogether rather faint). Before this stain, I prepared fresh thiosulfate buffer, and formaldehyde was freshly added to the silver solution and the developing solution.
What can be the cause of the inverse staining, an thus, how can I get back positive stains?

Thanks for helping me,


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