silver stain problem

beismann at em.uni-frankfurt.de beismann at em.uni-frankfurt.de
Thu Oct 10 09:24:32 EST 2002


Hello,

I have done silver stains of SDS gels many times before but last time something strange occured: I got an inverse stain with dark background and light protein bands (altogether rather faint). Before this stain, I prepared fresh thiosulfate buffer, and formaldehyde was freshly added to the silver solution and the developing solution.
What can be the cause of the inverse staining, an thus, how can I get back positive stains?

Thanks for helping me,

Silke



http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0



More information about the Proteins mailing list