silver stain problem

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Thu Oct 10 21:56:48 EST 2002


Most likely poor wash in between changes of reagents, or you have
inadvertently heated the gel at the developing stage.

A.G.E.
<beismann at em.uni-frankfurt.de> wrote in message
news:20021010142432.2660.qmail at ww02.hostica.com...
> Hello,
>
> I have done silver stains of SDS gels many times before but last time
something strange occured: I got an inverse stain with dark background and
light protein bands (altogether rather faint). Before this stain, I prepared
fresh thiosulfate buffer, and formaldehyde was freshly added to the silver
solution and the developing solution.
> What can be the cause of the inverse staining, an thus, how can I get back
positive stains?
>
> Thanks for helping me,
>
> Silke
>
>
>
>
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