buffer and metals

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Thu Oct 10 22:01:52 EST 2002

I am assuming near-neutral pH is needed ? Both Tris and Hepes are OK for Cu
and Zn. Amines and ammonia-based buffers might form complexes - especially
with copper, phosphate precipitates with many divalent metals - technically
acetate forms weak complexes too but that's usually not a problem for
biological applications. Citrate isn't a good choice, especially with
divalent metal ions and so's carbonate. Many inorganic chemistry books
contain salt solubility charts - if you're not sure what's going to happen
in a particular case, you can look up solubilities of salts & complexes for
many common buffering agents and metals.

"MASSIMILIANO COMAI" <massimiliano.comai at tin.it> wrote in message
news:7fVo9.24098$Fz.700458 at news1.tin.it...
> I must work with  peptides in presence of metals (Copper and zinc) but who
> knows a good buffer that not form complexes with those metals??
> Hepes or Tris are good or not?
> Thanks
> Max

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