silver stain problem

janosch groening janosch.groening at
Fri Oct 11 11:17:44 EST 2002

Hello A.G.E.,
as coincidence works, I did just yesterday for the first time the "Quick&dirty"
silver staining method (microwave) which you mentioned here a few months back.
I had two SDS-PAGE's, one stained with "my" usual method (3h) and the other one
with the quick method (30min). It worked really great, at the first approach.
And there were not such _big_ differences between the quick and the "slow"
Actually, though, I did first the step with the thiosulfate and then the
staining-step with the silver, as "my" "old" method is proceeding like that.
just wanted to take the advantage to "report" this, seeing your post today ;).
with kindest regards

Artem Evdokimov schrieb:

> Most likely poor wash in between changes of reagents, or you have
> inadvertently heated the gel at the developing stage.
> A.G.E.
> <beismann at> wrote in message
> news:20021010142432.2660.qmail at
> > Hello,
> >
> > I have done silver stains of SDS gels many times before but last time
> something strange occured: I got an inverse stain with dark background and
> light protein bands (altogether rather faint). Before this stain, I prepared
> fresh thiosulfate buffer, and formaldehyde was freshly added to the silver
> solution and the developing solution.
> > What can be the cause of the inverse staining, an thus, how can I get back
> positive stains?
> >
> > Thanks for helping me,
> >
> > Silke
> >
> >
> >
> >
-------------- next part --------------
A non-text attachment was scrubbed...
Name: janosch.groening.vcf
Type: text/x-vcard
Size: 265 bytes
Desc: Visitenkarte für janosch groening
Url :

More information about the Proteins mailing list