why phenol dissolves proteins better
Zhang at mpimp-golm.mpg.de
Fri Oct 11 11:56:40 EST 2002
I appreciate to knowing the chemical reasons,
From: janosch groening [mailto:janosch.groening at ioez.tu-freiberg.de]
Sent: Freitag, 11. Oktober 2002 18:18
To: proteins at hgmp.mrc.ac.uk
Subject: Re: silver stain problem
as coincidence works, I did just yesterday for the first time the "Quick&dirty"
silver staining method (microwave) which you mentioned here a few months back.
I had two SDS-PAGE's, one stained with "my" usual method (3h) and the other one
with the quick method (30min). It worked really great, at the first approach.
And there were not such _big_ differences between the quick and the "slow"
Actually, though, I did first the step with the thiosulfate and then the
staining-step with the silver, as "my" "old" method is proceeding like that.
just wanted to take the advantage to "report" this, seeing your post today ;).
with kindest regards
Artem Evdokimov schrieb:
> Most likely poor wash in between changes of reagents, or you have
> inadvertently heated the gel at the developing stage.
> <beismann at em.uni-frankfurt.de> wrote in message
> news:20021010142432.2660.qmail at ww02.hostica.com...
> > Hello,
> > I have done silver stains of SDS gels many times before but last time
> something strange occured: I got an inverse stain with dark background and
> light protein bands (altogether rather faint). Before this stain, I prepared
> fresh thiosulfate buffer, and formaldehyde was freshly added to the silver
> solution and the developing solution.
> > What can be the cause of the inverse staining, an thus, how can I get back
> positive stains?
> > Thanks for helping me,
> > Silke
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