silver stain problem

Peter Deckers peter.deckers at ua.ac.be
Mon Oct 14 06:01:55 EST 2002


FYI

I  stained a 2D gel with sypro ruby and afterwards with silver staining and
I got the same effect ...
Strange eh

<beismann at em.uni-frankfurt.de> wrote in message
news:20021010142432.2660.qmail at ww02.hostica.com...
> Hello,
>
>
>
> I have done silver stains of SDS gels many times before but last time
something strange occured: I got an inverse stain with dark background and
light protein bands (altogether rather faint). Before this stain, I prepared
fresh thiosulfate buffer, and formaldehyde was freshly added to the silver
solution and the developing solution.
>
> What can be the cause of the inverse staining, an thus, how can I get back
positive stains?
>
>
>
> Thanks for helping me,
>
>
>
> Silke
>
>
>
>
>
>
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