brush border membrane vesicle preparation
Dr Engelbert Buxbaum
engelbert_buxbaum at web.de
Tue Oct 15 05:35:16 EST 2002
teerachild at yahoo.com wrote:
> Hi, I just wanna know something about brush border membrane vesicle preparation (BBMV). Can I purifiy BBMV by boiling of insect guts in SDS-PAGE sample buffer ?
This would give a preparation which would also include proteins from a
lot of other compartments (gut proteins rather than brush border), all
of which would be denatured and non-functional. They would not be in
vesicles (closed membrane fragments) but in detergent micelles.
The usual procedure would be to homogenise the tissue (Potter-Elveijhem)
and to isolate the brush border membranes by a series of cenrifugation
steps. Use marker enzymes to characterise your fractions.
Check books on basic biochemical techniques for further info, "Methods
in Enzymology" for specific methods.
More information about the Proteins