PhastSystem IEF

Rodrigo Arteaga eceh1 at
Wed Oct 16 11:56:14 EST 2002

Hi, maybe a silly question but my experience in Proteins is poor.

I need to perform a IEF of my bacterial extract (semipurified) but I
do not which is the best buffer to use with my sample, I have
precipitated my sample (acetone) and resuspended with H2Od but the
sample doesn´t run in the gel, it stay in the same place (the standars
run well) no matter where I put the sample (acid or basic side)(using
Phast system gel).

Thanks in advence.

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