PhastSystem IEF

[Artem Evdokimov]

> I need to perform a IEF of my bacterial extract (semipurified) but I
> do not which is the best buffer to use with my sample, I have
> precipitated my sample (acetone) and resuspended with H2Od but the
> sample doesn´t run in the gel, it stay in the same place (the standars
> run well) no matter where I put the sample (acid or basic side)(using
> Phast system gel).

One of the possibilities is that you probably denatured and
super-crosslinked your protein(s). Can you work with non-denatured material
?

A.G.E.