High school protein purification lab
Dr Engelbert Buxbaum
engelbert_buxbaum at web.de
Fri Oct 18 03:25:49 EST 2002
jculp at egreen.wednet.edu wrote:
> I am looking for a new protein purification lab for my high school students. Our equipment and supplies are limited. We have a couple of specs and ability to do chromatography. Does anyone have any suggestions? I understand that this is very general question. Please let me know if you have any thoughts on this.
Alcohol dehydrogenase from yeast is a very sturdy and abundant protein.
Purification is easy (aceton precipitation, heat denaturing, ion
exchange chromatography, ammonium sulfate crystallisation).
Activity is checked in a coupled spectrophotometric assay, which is an
important concept in itself.
ADH is a tetramere, so you can exemplify the differeences between native
and denaturing electrophoresis to.
More information about the Proteins