Degenerate PCR primer design software

Chang Zhu czhu at
Sat Oct 19 20:18:13 EST 2002

Primo Degenerate designs PCR primers based on a single protein
or multiple alignments of proteins or DNAs. The melting temperature
may be calculated using the nearest neighbor model, which
automatically corrects for the effective primer concentration for a
degenerate primer. The online version
is available at

Comments and suggestions are especially welcome.



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