Degenerate PCR primer design software

[Chang Zhu]

Primo Degenerate designs PCR primers based on a single protein
sequence
or multiple alignments of proteins or DNAs. The melting temperature
may be calculated using the nearest neighbor model, which
automatically corrects for the effective primer concentration for a
degenerate primer. The online version
is available at 

http://www.changbioscience.com/primo/primod.html

Comments and suggestions are especially welcome.

Thanks.

Chang