Ask help for protein purification using His-tag column!

[chengzhjun at hotmail.com]

    my protein is about 30kDa and its pI is 5.1. I used pH=8.0 PBS binding buffer to banlance the Ni-His tag column. but when i put the protein solution into the column,the protein solution becomes turbid and the resin aggregates together and so the protein  buffer could't flow down. How can I do  with it .
   Any information would be appreciated!
  Carol


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