Ask help for protein purification using His-tag column!

Pier Carlo Montecucchi pcmontecucchi at
Tue Oct 22 04:08:11 EST 2002

try prep. isoelectric focusing pH range 4 to 6 for ex.


Pier Carlo


Pier Carlo Montecucchi
<chengzhjun at> wrote in message
news:20021022055838.24989.qmail at
>     my protein is about 30kDa and its pI is 5.1. I used pH=8.0 PBS binding
buffer to banlance the Ni-His tag column. but when i put the protein
solution into the column,the protein solution becomes turbid and the resin
aggregates together and so the protein  buffer could't flow down. How can I
do  with it .
>    Any information would be appreciated!
>   Carol

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