histidine tagging

Nora Plesofsky nora at biosci.cbs.umn.edu
Tue Oct 29 15:20:48 EST 2002


I wish to tag proteins that I have engineered to be expressed in my 
organism. It seems to me that hexahistidine tagging would serve two 
purposes, i.e. allowing visualization of the protein by immunoblotting 
and also affinity isolation of the protein with attendant proteins. I 
am asking if others have had experience with antibody reactivity to the 
histidine tag and with background reactivity. Is there another tag that 
has served this purpose possibly better?

Thanks for your help.

Nora Plesofsky
nora at biosci.cbs.umn.edu

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