His-tag purification

c9520071 at hotmail.com c9520071 at hotmail.com
Thu Oct 31 08:58:38 EST 2002


has anybody an idea how i can get rid of background when i do his-tag purification out of an in vitro translation from e.coli extract using magnetic beads from quiagen?
i need the protein to be as clean as possible for further processing with MALDI-TOF

thx for ideas,



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