stripping and western blot?

Frank Fürst ffrank at rz.uni-potsdam.de
Sun Sep 1 03:48:30 EST 2002


dk at no.email.thankstospam.net (D.K.) schrieb:

> "Emir Khatipov" <khatipovNO at NOuchicago.edu> wrote:
> >
> >"Schwabe, TME" <tatjana.schwabe at ruhr-uni-bochum.de> wrote in message
> >news:3D6F9974.9010707 at ruhr-uni-bochum.de...
> >> > Seems interesting. I never heard of using low pH in Westerns, although
> >it is
> >> > widely used elsewhere. The only thing I wonder is how you make glycine
> >> > buffer pH 1.9, when none of the conventional pH meters can accurately
> >> > measure such a value? Could you post you method or the source here?
> >> > Emir
> >
> >One can always buy ready-made glycine 1.9 or whatever from chemical
> >suppliers. I use glycine 1.5-2.5 in BIAcore experiments and buy the buffers
> >from BIAcore, though it is quite expensive (but !guaranteed quality! :-))
> >.... Still wonder how THEY measure pH1.9. 
> 
> I might have a glaring hole in my education but this is first time in 
> my life I hear about difficulties measuring pH ~ 1.5 with a properly 
> calibrated pH-meter. Is there a problem? What kind? Would you 
> similarly say that pH 12 is unreliable? 

At high pH you get an error because the electrode will respond to Na+ or
Li+ (it's called "Alkali-Fehler" in german, "alkali error" or something
like that). But I also have never heard of an error at low pH.

What is it about?

TIA, Frank
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