stripping and western blot?
ffrank at rz.uni-potsdam.de
Sun Sep 1 03:48:30 EST 2002
dk at no.email.thankstospam.net (D.K.) schrieb:
> "Emir Khatipov" <khatipovNO at NOuchicago.edu> wrote:
> >"Schwabe, TME" <tatjana.schwabe at ruhr-uni-bochum.de> wrote in message
> >news:3D6F9974.9010707 at ruhr-uni-bochum.de...
> >> > Seems interesting. I never heard of using low pH in Westerns, although
> >it is
> >> > widely used elsewhere. The only thing I wonder is how you make glycine
> >> > buffer pH 1.9, when none of the conventional pH meters can accurately
> >> > measure such a value? Could you post you method or the source here?
> >> > Emir
> >One can always buy ready-made glycine 1.9 or whatever from chemical
> >suppliers. I use glycine 1.5-2.5 in BIAcore experiments and buy the buffers
> >from BIAcore, though it is quite expensive (but !guaranteed quality! :-))
> >.... Still wonder how THEY measure pH1.9.
> I might have a glaring hole in my education but this is first time in
> my life I hear about difficulties measuring pH ~ 1.5 with a properly
> calibrated pH-meter. Is there a problem? What kind? Would you
> similarly say that pH 12 is unreliable?
At high pH you get an error because the electrode will respond to Na+ or
Li+ (it's called "Alkali-Fehler" in german, "alkali error" or something
like that). But I also have never heard of an error at low pH.
What is it about?
[Gesucht war ein MTA für Massenmailing]
Aus Stabilitätsgründen würde ich es allerdings vorziehen, wenn als
Server-OS nicht Debian-Linux sondern Windows-95 verwendet wird.
[Elmar Haneke in debian-user-de@]
More information about the Proteins