stripping and western blot?

D.K. dk at no.email.thankstospam.net
Sun Sep 1 20:42:24 EST 2002


ffrank at rz.uni-potsdam.de (Frank =?iso-8859-1?q?F=FCrst?=) wrote:
>dk at no.email.thankstospam.net (D.K.) schrieb:
>
>> I might have a glaring hole in my education but this is first time in 
>> my life I hear about difficulties measuring pH ~ 1.5 with a properly 
>> calibrated pH-meter. Is there a problem? What kind? Would you 
>> similarly say that pH 12 is unreliable? 
>
>At high pH you get an error because the electrode will respond to Na+ or
>Li+ (it's called "Alkali-Fehler" in german, "alkali error" or something
>like that). But I also have never heard of an error at low pH.

Yes, this effect is easy to understand. Although I doubt it is significant
at pH 12 - IIRC, most electrodes' specs claim linear response to 12. 

DK



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