stabilizing enzyme during storage

Peter Cherepanov peter.cherepanov at uz.kuleuven.ac.be
Mon Sep 2 03:01:05 EST 2002


>Now, can you tell me how to get rid of the glycerol after defrosting. Is
the only way to dialyse??
>
>
>Thx,
>Daniel

well, one usually adds glycerol when it does not interfere with activity.
Apart from that, normally, the protein is concentrated, so that diluting it
later for activity tests will minimize any effect.

(Concentrated enzymes are also more stable).

if you are so unlucky, that glycerol has to be removed try anion exchange
(or cation) - if you have got enough sample, of course. 1ml HiTrap columns
from Pharmacia are just perfect for this kind of job. Dialysis may be not a
good idea if your sample is not stable.

Peter

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