misaacs at dontspamme.student.usyd.edu.au
Mon Sep 2 20:26:54 EST 2002
"Vayuputra" <vayuputra369 at hotmail.com> wrote in
news:akuh6d$bvj$1 at charm.magnus.acs.ohio-state.edu:
> I try to dissolve this pellet in my loading buffer (SDS,
> 2-mercaptoethanol, bromophenol blue, etc) but I just can't. I make
> sure the pellet is as dry as possible (to remove any possibility of
> residual acetone).
I generally don't dry to dryness - I leave at RT until I can't smell
acetone anymore, then about another 10-15mins. It makes it easier to
resuspend. Also, concentration with acetone precips can be problematic to
resuspend at the best of times. Have you considered low mW cut-off spin
filters as a concentration option?
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