Acetone precipitation

Mitchell Isaacs misaacs at dontspamme.student.usyd.edu.au
Mon Sep 2 20:26:54 EST 2002


"Vayuputra" <vayuputra369 at hotmail.com> wrote in
news:akuh6d$bvj$1 at charm.magnus.acs.ohio-state.edu: 


> I try to dissolve this pellet in my loading buffer (SDS,
> 2-mercaptoethanol, bromophenol blue, etc) but I just can't.  I make
> sure the pellet is as dry as possible (to remove any possibility of
> residual acetone). 

I generally don't dry to dryness - I leave at RT until I can't smell 
acetone anymore, then about another 10-15mins. It makes it easier to 
resuspend. Also, concentration with acetone precips can be problematic to 
resuspend at the best of times. Have you considered low mW cut-off spin 
filters as a concentration option?

Cheers,
Mitchell



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